An acyl-SAM analog as an affinity ligand for identifying quorum sensing signal synthases.
نویسندگان
چکیده
N-Acylhomoserine lactones (AHLs) are quorum sensing signals produced by Gram-negative bacteria. We here report the affinity purification of AHL synthases using beads conjugated with an enzyme inhibitor, which was designed based on the catalytic intermediate acyl-SAM.
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 62 شماره
صفحات -
تاریخ انتشار 2014